제목 | [논문] 양시영 교수님_3´-Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental… | ||||
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작성자 | 비임파성 장기 면역 연구센터 | 등록일 | 2018-11-13 | 조회수 | 1552 |
3´-Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis
Abstract : 3´-Sialyllactose (3´-SL) is a safe compound that is present in high levels in human milk. Although it has anti-inflammatory properties and supports immune homeostasis, its effect on collagen-induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3´-SL on the progression of rheumatoid arthritis (RA) in in vitro and in vivo models. The anti-arthritic effect of 3´-SL was analysed with fibroblast-like synoviocytes in vitro and an in vivo mouse model of CIA. RT-PCR, Western blotting and ELISA were performed to evaluate its effects in vitro. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin-O and haematoxylin staining. 3´-SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated via blockade of the NF-κB signalling pathway. Therefore, 3´-SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA. |